Renal function during arterial hypertension produced by chronic splanchnic nerve stimulation in the dog.

Trained conscious mongrel dogs were used as the experimental animals. Arterial blood pressure was measured by means of a van Leersum carotid loop (3, 4). Renal plasma flow (RPF) and glomerular filtratration (GF) were determined by para-aminohippurate (PAH) and creatinine clearances, respectively. Filtration fraction (FF) was calculated from RPF and GF. Creatinine and PAH were administered intravenously by a constant-injection pump. Dosage was based on the surface area of the dog which was calculated by the Meeh-Rubner formula: surface area (M2) = I I ,z W-~7/IOo (W = weight in kg, M = meters). Priming dosages were : PAH, 385 mg/M2; creatinine, 1530 mg/M2. Sustaining dosages were: PAH, 5.75 mg/M2/min. ; creatinine, I 2 mg/M2/min. Plasma PAH and creatinine concentrations were maintained as nearly as possible at 2 mg yO and 12 mg %, respectively. Aft er a 2o-minute period for equilibration, urine samples were collected every 20 minutes by catheter and the bladder was flushed with three 30-cc aliquots of normal saline. Jugular vein blood samples were taken at the midpoint of the period. Plasma PAH determinations were made by the Bratton and Marshall method (5) as modified by Smith et al. (6), and plasma creatinine by the method of Folin and Wu (7). All clearances were performed on animals fasted approximately 18 hours. Five-hundred ml of water were given via stomach tube about 30 minutes before starting the clearance procedures. Renal plasma flow in cubic centimeters/minute/square meter was calculated from: RPF = C,V,/.go Cp TA, in which C, = (urine plus wash) concentration of PAH; V, = urine volume plus wash dilution (ml) ;